mouse anti-synapsin1  (Synaptic Systems)

Journal: Scientific Reports

Article Title: AIM2 promotes excitatory glutamate receptor expression by inhibiting STING and contributes to bone cancer pain in male mice

doi: 10.1038/s41598-024-83027-w

Figure Lengend Snippet: Expression of absent in melanoma 2 (AIM2) in the presynaptic membrane. ( A ) Double immunofluorescent staining of spinal cord slices with anti-AIM2 (red) and anti-Synapsin1 (rose red) antibodies in the sham, BCP, BCP + CON-Lentivirus (LV), and BCP + AIM2-LV groups. DAPI (blue) was used to stain the nucleus. The precise injection site of the virus in the dorsal horn was indicated by the green fluorescence, which represented the neuron transfected with lentivirus (n = 5). ( B ) Using ImageJ software, the percentages of AIM2 + and Synapsin-1 + co-localized volume in the total Synapsin-1 + volume in the presynaptic membrane of the spinal dorsal horn were quantified by analyzing five regions of each slice (* P < 0.05 vs. the sham; # P < 0.05 vs. the BCP + CON-LV; n = 5; mean ± SEM; one-way ANOVA followed by Tukey’s post-hoc test). n represents the number of experimental animals in each group.

Article Snippet: The antibodies used were as follow: mouse anti-AIM2 monoclonal antibody (1:300; cat. no.: 66902-1-IG, RRID: AB_2882230; Proteintech, USA) ; rabbit anti-AIM2 polyclonal antibody (1:300; cat. no.: bs-5986R, RRID: AB_11074508; Bioss, China) ; mouse anti-GluN1 monoclonal antibody (1:300; cat. no.: 32-0500, RRID: AB_86917; Thermo Fisher Scientific, USA) ; rabbit anti-STING polyclonal antibody (1:300; cat. no.: 19851-1-AP, RRID: AB_10665370; Proteintech, USA) ; mouse anti-Synapsin1 monoclonal antibody (1:300; cat. no.: MA5-31919, RRID: AB_2787542; Thermo Fisher Scientific, USA); guinea pig anti-MAP2 monoclonal antibody (1:300; cat. no.: 188004, RRID: AB_2138181; Synaptic Systems, Germany); mouse anti-Iba1 antibody (1:200; cat. no.: sc-32725, RRID: AB_667733; Santa Cruz, USA).

Techniques: Expressing, Membrane, Staining, Injection, Virus, Fluorescence, Transfection, Software

Journal: medRxiv

Article Title: Non-additive effects of schizophrenia risk genes reflect convergent downstream function

doi: 10.1101/2023.03.20.23287497

Figure Lengend Snippet:

Article Snippet: At day 21, cultures were fixed and immunostained as described previously, with an additional antibody stain for Synapsin1 (primary antibody: 1:500 Synapsin1 anti mouse (Synaptic Systems, 106 011); secondary antibody: donkey anti mouse Alexa 568 (Life technologies A10037)).

Techniques: